To remove all whitespaces in column MySQL there are several commands you can use.
To remove all whitespaces, you can using REPLACE query :
UPDATE `users` SET `username` = REPLACE(`username`, ' ', '');
To remove all tabs characters, use the following query :
UPDATE `users` SET `username` = REPLACE(`username`, '\t', '' );
To remove all new line characters, use the following query :
UPDATE `users` SET `username` = REPLACE(`username`, '\n', '');
To remove whitespaces from the beginning and end of column, you can using TRIM query :
UPDATE `users` SET `username` = TRIM(`username`);
Hopefully useful.